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mouse mab 9f10 (Developmental Studies Hybridoma Bank)

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Developmental Studies Hybridoma Bank mouse mab 9f10
Mouse Mab 9f10, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+9f10/mouse+antielav++9f10++antibody/pmc06080939-196-0-24
Average 90 stars, based on 1 article reviews

mouse mab 9f10 - by Bioz Stars, 2026-09

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Article Title: Integrin α4β7 switches its ligand specificity via distinct conformer-specific activation
Article Snippet: Mouse mAb 9F10 against human α4, rat mAb FIB504 against human β7, and rat mAb AIIB2 against human β1 were prepared by using hybridomas (Developmental Studies Hybridoma Bank).

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UBE2G2 and UBE2J2 regulate US2-induced immunoreceptor downregulation. (A,B) U937 (A) or THP-1 (B) cells expressing US2 were lentivirally transduced with a CRISPR/Cas9 vector targeting TRC8 (gRNA #1), UBE2G2 (gRNA #1) or UBE2J2 (gRNA #1). At 2 days after infection, gRNA-expressing cells were selected using puromycin. Cell surface expression of integrin-α4 (ITGA4), thrombomodulin (THBD) and HLA-I (HLA-A2 for THP-1 cells, HLA-A3 for U937 cells) was assessed by flow cytometry at 10 days (U937) or 15 days (THP-1) post infection. (C) THP-1 cells expressing US2 were lentivirally transduced with two CRISPR gRNAs (#4 and #5) targeting UBE2G2. At 2 days after infection, gRNA-expressing cells were selected using puromycin, and expression of integrin α1 (ITGA1), integrin α2 (ITGA2) or IL12 receptor β1-subunit (IL12R-B1) was assessed by flow cytometry at 7 dpi. (D) Lysates from cells used in B were prepared and subjected to immunoblotting analysis for total protein expression levels of ITGA2, ITGA4, thrombomodulin, and HLA-I (HCA2). US11-expressing cells were included as a control. Actin was used as a loading control. The asterisk marks an unspecific band.

Journal: Journal of Cell Science

Article Title: Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation

doi: 10.1242/jcs.206839

Figure Lengend Snippet: UBE2G2 and UBE2J2 regulate US2-induced immunoreceptor downregulation. (A,B) U937 (A) or THP-1 (B) cells expressing US2 were lentivirally transduced with a CRISPR/Cas9 vector targeting TRC8 (gRNA #1), UBE2G2 (gRNA #1) or UBE2J2 (gRNA #1). At 2 days after infection, gRNA-expressing cells were selected using puromycin. Cell surface expression of integrin-α4 (ITGA4), thrombomodulin (THBD) and HLA-I (HLA-A2 for THP-1 cells, HLA-A3 for U937 cells) was assessed by flow cytometry at 10 days (U937) or 15 days (THP-1) post infection. (C) THP-1 cells expressing US2 were lentivirally transduced with two CRISPR gRNAs (#4 and #5) targeting UBE2G2. At 2 days after infection, gRNA-expressing cells were selected using puromycin, and expression of integrin α1 (ITGA1), integrin α2 (ITGA2) or IL12 receptor β1-subunit (IL12R-B1) was assessed by flow cytometry at 7 dpi. (D) Lysates from cells used in B were prepared and subjected to immunoblotting analysis for total protein expression levels of ITGA2, ITGA4, thrombomodulin, and HLA-I (HCA2). US11-expressing cells were included as a control. Actin was used as a loading control. The asterisk marks an unspecific band.

Article Snippet: Primary antibodies used in our studies were: mouse anti-HLA-I HC HC10 monoclonal antibody (mAb), 1:400; mouse anti-HLA-I HC HCA2 mAb, 1:25; phycoerythrin (PE)-conjugated mouse anti-HLA-A2 mAb (clone BB7.2, no. 558570, BD Pharmingen), 1:20; human anti-HLA-A3 OK2F3 mAb (LUMC, Leiden, the Netherlands), 1:40; mouse anti-TfR H68.4 mAb (no. 13-68xx, Invitrogen), 1:5000; mouse anti-FLAG-M2 mAb (no. F1804, Sigma-Aldrich), 1:10,000; rat anti-HA 3F10 mAb (no. 11867423001, Roche) 1:1000; rabbit anti-UBE2G2 mAb (EPR9248, no. ab174296, Abcam), 1:1000; rabbit anti-TRC8 polyclonal antibody (pAb) (H89, no. sc-68373, Santa Cruz Biotechnology), 1:1000; mouse anti-CD141 (THBD) mAb (clone 1A4, no. 559780, BD Pharmingen), 1:320; mouse anti-CD49b (ITGA2) mAb (clone 12F1, no. 555668, BD Pharmingen), 1:1000; mouse anti-CD49d (ITGA4) mAb (clone 9F10, no. 555502, BD Pharmingen), 1:1000; rabbit anti-ITGA4 mAb (EPR1355Y, no. ab81280, Abcam), 1:1000; mouse anti-IL12 receptor β1 subunit (CD212) mAb (clone 2.4E6, no. 556064, BD Pharmingen), 1:1000; and rabbit anti-ITGA2 mAb ( {"type":"entrez-protein","attrs":{"text":"EPR17338","term_id":"523383441","term_text":"EPR17338"}} EPR17338 , no. ab181548, Abcam), 1:1000.

Techniques: Expressing, Transduction, CRISPR, Plasmid Preparation, Infection, Flow Cytometry, Western Blot